ゲノムネットワークプロジェクト

個別生命機能の解析
研究課題 エピジェネティックネットワークを介した幹細胞維持の分子機序
研究期間 平成18年度〜20年度
研究課題代表者 古関 明彦
所属機関 理化学研究所 免疫・アレルギー科学総合研究センター
研究目的 幹細胞維持には、ポリコム群をはじめとする内因性のエピジェネティック制御だけではなく、外因性シグナル群も重要な役割を果たすことはすでに明らかにされているが、これらがどのように相互作用するのかについては、おそらくヒストン修飾を介した相互作用が存在すること以外に多くのことは知られていない。
研究概要 本研究は、幹細胞システムに焦点を絞り、エピジェネティックシステムと外因性シグナル群のそれぞれが構成するネットワークの機能的相関を明らかにすることを目的とする。そのために、①胚性幹細胞(ES細胞)をモデルとして用いた多能性維持に必要なエピジェネティックネットワークの解明、②生体内の組織幹細胞におけるクロマチン状態を解析するための実験システムの構築、の2点に焦点を絞った研究を行う。
研究成果 ES細胞維持に作用する転写因子群が形成するコアネットワークは、エピジェネティックシステムのひとつであるポリコム群を利用して、発生や分化に関連する遺伝子群の発現を抑制し、未分化性を維持していることが新たに明らかになった。また、生体内の特定の細胞においてのみ、ポリコム群タンパクをビオチン化する実験システムの開発に成功した。
データ種別 Microarray data (Affymetrix), ChIP-chip data (Agilent)
対象生物 マウス
組織・細胞株 embryo(tissue), testis(germ cell)
対象遺伝子 -
実験情報 アレイ名称: Mouse430 2.0 Array
産出データ
(ダウンロード)

全産出データのダウンロード(FTP)

・マウスの幹細胞に対するマイクロアレイ実験の生データ
・実験に利用した細胞サンプルの詳細情報
XMLデータ -
研究論文・特許出願・
学術発表など
------------------------------ PUBLICATION ------------------------------
PUBLICATION ID:732
PUBLICATION TYPE: journal
PUBLICATION NAME: Nature
ARTICLE TITLE: The SRA protein Np95 mediates epigenetic inheritance by recruiting Dnmt1 to methylated DNA
SUBMIT DATE:
ACCEPT DATE:
PUBLICATION DATE: 2007-12-01
TITLE: The SRA protein Np95 mediates epigenetic inheritance by recruiting Dnmt1 to methylated DNA
STATUS: Published
AUTHOR NAME: Jafar Sharif1,2,*, Masahiro Muto3,*, Shin-ichiro Takebayashi4,*, Isao Suetake5, Akihiro Iwamatsu6, Takaho A. Endo7, Jun Shinga3, Yoko Mizutani-Koseki3, Kunihiro Okamura2, Shoji Tajima5, Kohzoh Mitsuya8,, Masaki Okano4,, and Haruhiko Koseki3,
AUTHOR AFFILIATION: 1Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan 2Department of Obstetrics and Gynecology, Tohoku University School of Medicine, 1-1 Seiryo-machi, Aoba-ku, Sendai 980-8574, Japan 3RIKEN Research Center for Allergy and Immunology, 1-7-22 Suehiro, Tsurumi-ku, Yokohama 230-0045, Japan 4RIKEN Center for Developmental Biology, 2-2-3 Minatojima-minamimachi, Chuo-ku, Kobe, Hyogo 650-0047, Japan 5Institute for Protein Research, Osaka University, 3-2 Yamadaoka, Suita, Osaka 565-0871, Japan 6Protein-Research Network, Inc., 1-13-5 Fukuura, Kanazawa-ku, Yokohama 236-0004, Japan 7RIKEN Genomic Sciences Center, 1-7-22 Suehiro, Tsurumi-ku, Yokohama 230-0045, Japan 8Biofunctional Science, Tohoku University Biomedical Engineering Research Organization (TUBERO), 2-1 Seiryo-machi, Aoba-ku, Sendai 980-8575, Japan *These authors contributed equally to this work.

------------------------------ PUBLICATION ------------------------------
PUBLICATION ID:1154
PUBLICATION TYPE: journal
PUBLICATION NAME: Nature Cell Biology
ARTICLE TITLE: Ring1-mediated ubiquitination of H2A restrains poised RNA polymerase II at bivalent genes in mouse ES cells
SUBMIT DATE:
ACCEPT DATE:
PUBLICATION DATE: 2007-12-01
TITLE: Ring1-mediated ubiquitination of H2A restrains poised RNA polymerase II at bivalent genes in mouse ES cells
STATUS: Published
AUTHOR NAME: Julie K. Stock1,6, Sara Giadrossi2,6, Miguel Casanova3, Emily Brookes1, Miguel Vidal4, Haruhiko Koseki5, Neil Brockdorff3, Amanda G. Fisher2, Ana Pombo1
AUTHOR AFFILIATION: 1Nuclear Organisation, 2Lymphocyte Development and 3Developmental Epigenetics Groups, MRC Clinical Sciences Centre, Imperial College School of Medicine, Hammersmith Hospital Campus, Du Cane Road, London W12 0NN, UK. 4Department of Developmental and Cell Biology, Centro de Investigaciones Biológicas, CSIC, Madrid, Spain. 5Department of Developmental Genetics, RIKEN Research Center for Allergy and Immunology, RIKEN Yokohama Institute, Yokohama, Japan. 6These authors contributed equally to this work.

------------------------------ PUBLICATION ------------------------------
PUBLICATION ID:1410
PUBLICATION TYPE: journal
PUBLICATION NAME: Development
ARTICLE TITLE: Mammalian Polycomb Scmh1 mediates exclusion of Polycomb complexes from the XY body in the pachytene spermatocytes
SUBMIT DATE:
ACCEPT DATE:
PUBLICATION DATE: 2007-02-01
TITLE: Mammalian Polycomb Scmh1 mediates exclusion of Polycomb complexes from the XY body in the pachytene spermatocytes
STATUS: Published
AUTHOR NAME: Takada Y, Isono K, Shinga J, Turner JM, Kitamura H, Ohara O, Watanabe G, Singh PB, Kamijo T, Jenuwein T, Burgoyne PS, Koseki H
AUTHOR AFFILIATION: RIKEN Research Center for Allergy and Immunology, 1-7-22 Suehiro, Tsurumi-ku, Yokohama 230-0045, Japan. Division of Stem Cell Research and Developmental Genetics, MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK. Laboratory of Veterinary Physiology, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183-8509, Japan. Nuclear Reprogramming Laboratory, Division of Gene Expression and Development, Roslin Institute (Edinburgh), Roslin, Midlothian EH25 9PS, UK. Department of Pediatrics, Shinshu University School of Medicine, Matsumoto, Nagano 390-8621, Japan. Research Institute of Molecular Pathology, The Vienna Biocenter, Dr Bohrgasse 7, A-1030 Vienna, Austria.

------------------------------ PUBLICATION ------------------------------
PUBLICATION ID:1475
PUBLICATION TYPE: journal
PUBLICATION NAME: Development
ARTICLE TITLE: Polycomb group proteins Ring1A/B are functionally linked to the core transcriptional regulatory circuitry to maintain ES cell identity
SUBMIT DATE:
ACCEPT DATE:
PUBLICATION DATE: 2008-04-01
TITLE: Polycomb group proteins Ring1A/B are functionally linked to the core transcriptional regulatory circuitry to maintain ES cell identity
STATUS: Published
AUTHOR NAME: Endoh M, Endo TA, Endoh T, Fujimura Y, Ohara O, Toyada T, Otte AP, Okano M, Brockdorff N, Vidal M, Koseki H.
AUTHOR AFFILIATION: RIKEN Research Center for Allergy and Immunology, 1-7-22 Suehiro, Tsurumi-ku, Yokohama 230-0045, Japan. RIKEN Genomic Sciences Center, 1-7-22 Suehiro, Tsurumi-ku, Yokohama 230-0045, Japan. Swammerdam Institute for Life Sciences, University of Amsterdam, Kruislaan 406, 1098 SM Amsterdam, The Netherlands. RIKEN Center for Developmental Biology, 2-2-3 Minatojima-minamimachi, Chuo-ku, Kobe, Hyogo 6500047, Japan. Developmental Epigenetics Group, MRC Clinical Sciences Centre, ICFM, Hammersmith Hospital, DuCane Road, London W12 ONN, UK. Centro de Investigaciones Biologicas, Department of Developmental and Cell Biology, Ramiro de Maeztu 9, 28040 Madrid, Spain.

------------------------------ PUBLICATION ------------------------------
PUBLICATION ID:1476
PUBLICATION TYPE: journal
PUBLICATION NAME: Nature Genetics
ARTICLE TITLE: PRC1 and Suv39h specify parental asymmetry at constitutive heterochromatin in early mouse embryos.
SUBMIT DATE:
ACCEPT DATE:
PUBLICATION DATE: 2008-04-01
TITLE: PRC1 and Suv39h specify parental asymmetry at constitutive heterochromatin in early mouse embryos.
STATUS: Published
AUTHOR NAME: Mareike Puschendorf1, Rémi Terranova1, Erwin Boutsma2, Xiaohong Mao3,6, Kyo-ichi Isono4, Urszula Brykczynska1, Carolin Kolb1, Arie P Otte5, Haruhiko Koseki4, Stuart H Orkin3, Maarten van Lohuizen2 & Antoine H F M Peters1
AUTHOR AFFILIATION: 1. Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, CH-4058 Basel, Switzerland. 2. Division of Molecular Genetics and Centre for Biomedical Genetics, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands. 3. Department of Pediatric Oncology, Dana Farber Cancer Institute, Harvard Stem Cell Institute and Howard Hughes Medical Institute, Harvard Medical School, Boston, Massachusetts 02115, USA. 4. RIKEN Research Center for Allergy and Immunology, RIKEN Yokohama Institute, 1-7-22 Suehiro-cho, Tsurumi-ku Yokohama City, Kanagawa 230-0045, Japan. 5. Swammerdam Institute for Life Sciences, University of Amsterdam, Kruislaan 406, 1098 SM Amsterdam, The Netherlands. 6. Present address: Novartis Institutes for BioMedical Research, Inc., 250 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA.

------------------------------ PUBLICATION ------------------------------
PUBLICATION ID:1477
PUBLICATION TYPE: journal
PUBLICATION NAME: Molecular and Cellular Biology
ARTICLE TITLE: Inactivation of the polycomb group protein Ring1B unveils an antiproliferative role in hematopoietic cell expansion and cooperation with tumorigenesis associated with Ink4a deletion
SUBMIT DATE:
ACCEPT DATE:
PUBLICATION DATE: 2008-02-01
TITLE: Inactivation of the polycomb group protein Ring1B unveils an antiproliferative role in hematopoietic cell expansion and cooperation with tumorigenesis associated with Ink4a deletion
STATUS: Published
AUTHOR NAME: Carmela Calés,2 Mónica Román-Trufero,1 Leticia Pavón,2 Iván Serrano,2 Teresa Melgar,1 Mitsuhiro Endoh,3 Claudia Pérez,4 Haruhiko Koseki,3 and Miguel Vidal
AUTHOR AFFILIATION: Centro de Investigaciones Biológicas, Consejo Superior de Investigaciones Científicas, Ramiro de Maeztu 9, 28004 Madrid, Spain,1 Instituto de Investigaciones Biomédicas, Universidad Autónoma de Madrid, Consejo Superior de Investigaciones Científicas, Arturo Duperier 4, 28029 Madrid, Spain,2 Riken Research Center for Allergy and Immunology, 1-7-22 Suehiro, Tsurumi-ku, Yokohama 230-0045, Japan,3 Anatomy and Histopathology, Facultad de Veterinaria, Universidad de León, Campus Vegazana, 24071 León, Spain4

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